CORN BRAN AS POTENTIAL SUBSTRATE FOR HIGH PRODUCTION OF FERULOYL AND ACETYLXYLAN ESTERASES BY SOLID STATE FERMENTATION
The aim of this study was to investigate some carbohydrate esterases production from a filamentous fungus grown by solid state fermentation on corn bran as substrate, mixed with polyurethane as support (75:25, w:w), and its comparison
with other agro-industrial by-products (wheat bran and coffee pulp) in the same conditions. Aspergillus niger, a known producer of feruloyl and other carbohydrate esterases, was used as fungal model. Cultures with corn bran induced a high
production of feruloyl esterase type A and B activity: 5.27 and 0.66 U gˉⁱ of dry matter over methyl ferulate and caffeate hydrolysis, respectively. Furthermore, in cultures with coffee pulp it is selectively induced the production of feruloyl
esterase B activity compared to other by-products used. A zymographic analysis of extracts of cultures on corn and wheat bran revealed another enzyme, later identified as acetylxylan esterase A, capable to hydrolyze methyl hydroxycinnamates
and p-nitrophenyl acetate. Activity on p-nitrophenyl acetate was 33.4 U gˉⁱ of dry matter on cultures grown on corn bran. This work is the first report of high production of feruloyl and acetylxylan esterases using corn bran as substrate, which are
enzymes of increasing biotechnological interest.
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