GENETIC IDENTIFICATION OF THE BIOANODE AND BIOCATHODE OF A MICROBIAL ELECTROLYSIS CELL

  • R. Valdez-Ojeda Unidad de Energía Renovable. Centro de Investigación Científica de Yucatán. (CICY)
  • M. Aguilar-Espinosa Unidad de Bioquímica y Biología Molecular de Plantas
  • L. Gómez-Roque Unidad de Energía Renovable. Centro de Investigación Científica de Yucatán. (CICY)
  • B. Canto-Canché Unidad de Biotecnología
  • RM. Escobedo Gracia-Medrano Unidad de Bioquímica y Biología Molecular de Plantas
  • J. Domínguez-Maldonado
  • L. Alzate-Gaviria Unidad de Energía Renovable. Centro de Investigación Científica de Yucatán. (CICY)
Keywords: exoelectrogenic bacteria, hydrogen generation, Rhodopseudomonas palustris, hydrogenase, MEC

Abstract

The aim of this study was to identify the microorganisms present on the graphite cloth of the bioanode and biocathode of a Microbial Electrolysis Cell (MEC) using 16S ribosomal RNA gene cloning and sequencing. The results obtained indicated that the bioanode clones were related with the phyla: Firmicutes (15.3%), Proteobacteria (7.6%), Bacteroidetes (30.7%), and Ignavibacteriae (7.6%). Conversely, the biocathode clones were related with the phylum Proteobacteria (38.4%). The bioanode clones were related with species identified previously in MECs and Microbial Fuel Cells (MFCs). However, the biocathode clones were related with Rhodopseudomonas palustris, which have not been reported for hydrogen production by MEC. R. palustris probably should be involved into hydrogen production of 0.011 m3 H2 /m3 cathode liquid volume per day with an applied voltage of 1 V.

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Published
2020-02-17
How to Cite
Valdez-Ojeda, R., Aguilar-Espinosa, M., Gómez-Roque, L., Canto-Canché, B., Escobedo Gracia-Medrano, R., Domínguez-Maldonado, J., & Alzate-Gaviria, L. (2020). GENETIC IDENTIFICATION OF THE BIOANODE AND BIOCATHODE OF A MICROBIAL ELECTROLYSIS CELL. Revista Mexicana De Ingeniería Química, 13(2), 573-581. Retrieved from http://www.rmiq.org/ojs311/index.php/rmiq/article/view/1352
Section
Biotechnology

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