• J. De Neve Department of Mathematical Modelling, Statistics and Bioinformatics, Ghent University
  • G. Goswami Department of Biotechnology, National Institute of Technology, Durgapur
  • A. Dutta Faculteit Industriële Ingenieurswetenschappen, KU Leuven, Campus Leuven (@Groep T)
  • S. Chaudhuri
  • D. Dutta Department of Biotechnology, National Institute of Technology, Durgapur
Keywords: Dietzia maris NIT-D, biomass, canthaxanthin, response surface methodology


Dietzia maris NIT-D, a producer of canthaxanthin, is isolated during routine screening of pigment producing bacteria. The effects of process parameters, namely temperature, pH, shaker speed, percentage inoculum, medium volume, and concentration of glucose on the canthaxanthin production are studied by using response surface design methodology. The optimal conditions are temperature = 30◦C, pH = 5.9, shaker speed = 125 rpm, inoculum = 1.9 %, volume = 50 mL, and glucose = 15 g L−1, resulting in a canthaxanthin production of 152 mg L−1, which is 25% higher than that of a recently reported study.


Asker D. and Ohta Y. (1999) Production of canthaxanthin by extremely halophilic bacteria. Journal of Bioscience and Bioengineering 88, 617–621

Asker D. and Ohta Y. (2002) Production of canthaxanthin by Haloferax alexandrinus under non-aseptic conditions and a simple, rapid method for its extraction. Applied Microbiology and Biotechnology 58, 743–750

Borzenkov IA, Milekhina EI, Gotoeva MT, Rozanova EP and Belyaev SS (2006) The properties of hydrocarbon-oxidizing bacteria isolated from the oilfields of Tatarstan, Western Siberia, and Vietnam. Microbiology 75, 66–72

Calo P., de Miguel T., Sieiro C., Velazquez JB and Villa TG (1995) Ketocarotenoids in halobacteria: 3-hydroxy echinenone and transastaxanthin. Journal of Applied Microbiology 79, 282–285

Cooney JJ and Berry RA (1981) Inhibition of carotenoid synthesis in Micrococcus roseus. Canadian Journal of Microbiology 27, 421–425

Corona-González, RI, Ramos-Ibarra, JR, Gutiérrez-González, P, Pelayo-Ortiz, C, Guatemala-Morales, GM and Arriola-Guevara, E. (2013) The use of response surface methodology to evaluate the fermentation conditions in the production of tepache. Revista Mexicana de Ingeniería Química 12, 19–28

Das A, Yoon S, Lee S, Kim J, Oh D and Kim S (2007) An update on microbial carotenoid production: application of recent metabolic engineering tools. Applied Microbiology and Biotechnology 77, 505–512

de Miguel T, Sieiro C, Poza M and Villa TG (2000) Isolation and taxonomic study of a new canthaxanthin-containing bacterium, Gordonia jacobaea MV-1 sp. nov. International Microbiology 3, 107–111

Don MM and Shoparwe NF (2010) Kinetics of hyarulonic acid production by Streptococcus zooepidemicus considering the effect of glucose. Biochemical Engineering Journal 49, 95–103

Duckworth AW, Grant S, Grant WD, Jones BE and Meijer D (1998) Dietzia natronolimnaios sp. nov., a new member of the genus Dietzia isolated from an East African soda lake. Extremophiles 2, 359–366

Gharibzahedi SMT, Razavi SH and Mousavi M (2013) Kinetic analysis and mathematical modelling of cell growth and canthaxanthin biosynthesis by Dietzia natronolimnaea HS-1 on waste molasses hydrolysate. RSC Advances 3, 23495–23502

Goswami G, Chakraborty S, Chaudhuri S and Dutta D (2012) Optimization of process parameters by response surface methodology and kinetic modeling for batch production of canthaxanthin by Dietzia maris NIT-D (accession number: M151403). Bioprocess and Biosystems Engineering 35, 1375–1388

Hannibal L, Lorquin J, D’Ortoli NA, García N, Chaintreuil C, Masson-Boivin C, Dreyfus B and Giraud E (2000) Isolation and characterization of canthaxanthin biosynthesis genes from the photosynthetic bacterium Bradyrhizobium sp. Strain ORS278. Journal of Bacteriology 182, 3850–3853

Khodaiyan F, Razavi SH, Emam-Djomeh Z and Mousavi SM (2007a) Optimization of canthaxanthin production by Dietzia natronolimnaea HS-1 using response surface methodology. Pakistan Journal of Biological Sciences 10, 2544–2552

Khodaiyan F, Razavi SH, Emam-Djomeh Z, Mousavi SM and Hejazi MA (2007b) Effect of culture conditions on canthaxanthin production by Dietzia natronolimnaea HS-1. Journal of Microbiology and Biotechnology 17, 195–201

Khodaiyan F, Razavi SH, Mousavi SM (2008) Optimization of canthaxanthin production by Dietzia natronolimnaea HS-1 from cheese whey using statistical experimental methods. Biochemical Engineering Journal 40, 415–422

Krupa D, Nakkeeran E, Kumaresan N, Vijayalakshmi G and Subramanian R (2010) Extraction, purification and concentration of partially saturated canthaxanthin from Aspergillus carbonarius. Bioresource Technology 101, 7598–7604

Kutner MH, Nachtsheim CJ, Neter J and Li W (2005) Applied Linear Statistical Models, 5th edn. McGraw-Hill, New York

Lorquin J, Molouba F and Dreyfus BL (1997) Identification of the carotenoid pigment canthaxanthin from photosynthetic Bradyrhizobium strains. Applied and Environmental Microbiology 63, 1151–1154

Malis, SA, Cohen E and Ben Amotz A (1993) Accumulation of canthaxanthin in Chlorella emersonii. Physiologia Plantarum 87, 232–236

Moller AP, Biard C, Blount JD, Houston DC, Ninni P, Saino N and Surai PF (2000) Carotenoiddependent signals: indicators of foraging efficiency, immunocompetence or detoxification ability? Avian and Poultry Biology Reviews 11, 137–159

Montgomery DC (2001) Design and Analysis of Experiments, 5th edn. Wiley, New York

Nelis JH and de Leenheer AP (1989) Reinvestigation of Brevibacterium sp. Strain KY-4313 as a source of canthaxanthin. Applied and Environmental Microbiology 55, 2505–2510

Padmapriya K, Dutta A, Chaudhuri S and Dutta D. (2012) Microwave assisted extraction of mangiferin from Curcuma amada. 3 Biotech 2, 27–30

R Core Team (2012) R: A Language and Environment for Statistical Computing (http://www.R-project.org), Vienna, Austria

Rodriguez-Marín ML, Bello-Perez LA, Yee-Madeira H and González–Soto RA (2013) Propiedades mecánicas y de barrera de películas elaboradas con harina de arroz y plátano reforzadas con nanopartículas: estudio con superficie de respuesta. Revista Mexicana de Ingeniería Química 12, 165–176

Veiga-Crespo P, Blasco L, Rosa dos Santos F, Poza M and Villa TG (2005) Influence of culture conditions of Gordonia jacobaea MV26 on canthaxanthin production. International Microbiology 8, 55–58
How to Cite
De Neve, J., Goswami, G., Dutta, A., Chaudhuri, S., & Dutta, D. (2020). A STATISTICALLY MOTIVATED CHOICE OF PROCESS PARAMETERS FOR THE IMPROVEMENT OF CANTHAXANTHIN PRODUCTION BY DIETZIA MARIS NIT-D (ACCESSION NUMBER: HM151403). Revista Mexicana De Ingeniería Química, 13(2), 595-603. Retrieved from http://www.rmiq.org/ojs311/index.php/rmiq/article/view/1355