• D.M. Palmerín-Carreño Departamento de Biotecnología, Universidad Autónoma Metropolitana
  • O.M. Rutiaga-Quiñones Departamento de Química-Bioquímica, Instituto Tecnológico de Durango
  • J.R. Verde-Calvo Departamento de Biotecnología, Universidad Autónoma Metropolitana
  • S. Huerta-Ochoa Departamento de Biotecnología, Universidad Autónoma Metropolitana
Keywords: bioconversion, ( )-nootkatone, Botryodiplodia theobromae, orange essential oil, membrane aerated biofilm reactor


The aim of this work was to evaluate the bioconversion of (+)-valencene to (+)-nootkatone by B. theobromae using a membrane aerated biofilm reactor (MABR) in a two liquid phase system with orange essential oil as the organic phase. In the aqueous phase system, a (+)-nootkatone production rate up to 3.98 mg L−1 h−1 was achieved, obtaining a final product concentration of 398.08 mg L−1 with a bioconversion of 62 %. A two liquid phase system, using orange essential oil as the dispersed phase, was also studied and a final (+)-nootkatone concentration of 310.37 mg L−1 was achieved in the organic phase, with a bioconversion of 30.5 % and a production rate of 2.46 mg L−1 day−1. The lower performance obtained using the two phase system was probably due to mass transfer limitations. The present work is the first report on an MABR for the bioconversion of (+)-valencene to (+)-nootkatone. Further studies on bioconversion products and optimization of biofilm reactor operations are needed to enhance bioconversion.


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How to Cite
Palmerín-Carreño, D., Rutiaga-Quiñones, O., Verde-Calvo, J., & Huerta-Ochoa, S. (2020). BIOCONVERSION OF (+)-NOOTKATONE BY Botryodiplodia theobromae USING A MEMBRANE AERATED BIOFILM REACTOR. Revista Mexicana De Ingeniería Química, 13(3), 757-764. Retrieved from

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