INFLUENCE OF THE TYPE OF SUPPORT AND IMMOBILIZATION ON THE ACTIVITY AND STABILITY OF LACCASE ENZYME (Trametes versicolor)

  • A. Castro Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa
  • I. González Departamento de Química, área de Electroquímica, Universidad Autónoma Metropolitana Unidad Iztapalapa
  • F. Tzompantzi Departamento de Química, área de catálisis, Universidad Autónoma Metropolitana Unidad Iztapalapa
  • G. Viniegra-González Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa
Keywords: laccase, immobilization, thiol, glassy carbon, hydrotalcite

Abstract

This work presents a comparative study of the influence of the nature of the substrate and the immobilization mechanism on laccase enzyme activity and stability. The supports employed were: hydrotalcite-like particles (ZnAl2), amorphous silica crystals, and glassy carbon surface. Two immobilization mechanisms were applied: one physical, by adsorption, and the other chemical, with two versions of covalent bonding. In the first, using silanes and glutaraldehyde (GA), in the second, thiols were used as anchoring reagents. Hydrotalcite and silica supports were characterized before and after immobilizing the enzyme by X-ray diffraction analysis (XRD), while in the case of glassy carbon supports electrochemical characterization was performed. The catalytic properties Km and Kcat/Km of every enzymatic system were evaluated in a complementary fashion, as well as the free enzyme. The kinetic characterizations were done using ABTS (ammonium 2,2’azino-bis-(3-ethylbenzothiazoline-6-sulfonate acid)), as a reagent typical of laccase in 0.1 M acetate buffer solution pH 3.7. Efficiency of the enzyme adsorbed on each support it was demonstrated that the method of immobilization is decisive in the catalysis. Greater efficiency was observed in the system that employed glassy carbon with either immobilization mechanism and these results are analogous with the free enzyme.

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Published
2020-03-18
How to Cite
Castro, A., González, I., Tzompantzi, F., & Viniegra-González, G. (2020). INFLUENCE OF THE TYPE OF SUPPORT AND IMMOBILIZATION ON THE ACTIVITY AND STABILITY OF LACCASE ENZYME (Trametes versicolor). Revista Mexicana De Ingeniería Química, 12(2), 241-255. Retrieved from http://www.rmiq.org/ojs311/index.php/rmiq/article/view/1464
Section
Biotechnology