EFFECT OF TEMPERATURE AND TWO DRYING PROCESSES ON THE INSECTICIDAL ACTIVITY OF A NUCLEOPOLYHEDROVIRUS OF Spodoptera frugiperda

  • J.E. Camacho Universidad de la Sabana,Universidad Colegio Mayor de Cundinamarca
  • M.I. Gómez Centro de Biotecnología y Bioindustria,Corpoica
  • L.F. Villamizar
Keywords: baculovirus, temperature, spray drying in fluid bed, oven drying, insecticidal activity

Abstract

The effectiveness and potential use of Baculovirus for controlling dierent insect pests have been shown in several studies. However, their evaluation under field conditions has presented inconsistent results, possibly because of the deleterious effect of environmental conditions such as temperature. Considering the potential of a Colombian isolate of Spodoptera frugiperda nucleopolyhedrovirus SfMNPV003 for developing a biopesticide, the objective of this study was to evaluate the effect of temperature and two drying processes over viral insecticidal activity, in order to use process conditions that allow to design effcient formulation. The nucleopolyhedrovirus, was exposed to temperatures between 3°C and 6°C for 30, 45, 60, 120 and 180 minutes. Processes tested were oven drying and spray drying in fluid bed, evaluating pH, moisture content, content of microbial contaminants, viral concentration, yield and insecticidal activity (LC50) of dry virus. The insecticidal activity of the virus decreased when it was exposed to temperatures above 5°C. Oven drying process was selected for presenting the highest yield, also because it did not aect the virus pathogenicity and allowed to obtain a dry powder with a lower microbial contaminants content.

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Published
2020-03-31
How to Cite
Camacho, J., Gómez, M., & Villamizar, L. (2020). EFFECT OF TEMPERATURE AND TWO DRYING PROCESSES ON THE INSECTICIDAL ACTIVITY OF A NUCLEOPOLYHEDROVIRUS OF Spodoptera frugiperda. Revista Mexicana De Ingeniería Química, 12(3), 437-450. Retrieved from http://www.rmiq.org/ojs311/index.php/rmiq/article/view/1514
Section
Biotechnology