• G. Ramírez-Romero
  • M. Reyes-Velazquez
  • A. Cruz-Guerrero
Keywords: bacteriocin, nejayote, probiotic


In the present work, the nejayote (nixtamalization residue from maize) as a culture medium for probiotic bacteria and production of bacteriocins was evaluated. Nejayotes obtained from three mills were characterized: reducing sugars 0.166-0.818 g/L, total sugars 23.57-63.41 g/L and protein 5.66-12.92 g/L. The fermentation was performed at 37◦C for 48 h by: Lactobacillus casei IMAU60214, Lactobacillus rhamnosus GG and Lactobacillus helveticus IMAU70129. Consumption of reducing sugars from 60-65%, total sugars from 6-30% and protein from 1-15% were observed. Furthermore, bacteriocin production was evaluated with the supernatants of fermentation. The greatest inhibition by the presence of bacteriocins was obtained with the supernatants of fermentation of Lactobacillus casei IMAU60214, and Lactobacillus helveticus IMAU70129. The highest inhibition was 14% for Listeria innocua ATCC33090 and 10% for Escherichia coli K-12. Bacteriocins presence was confirmed with addition of proteinase K. Since bacteriocins produced inhibited to L. innocua (Gram +) and E. coli (Gram -) can be said that they probably have a wide spectrum of inhibition. In conclusion, nejayote can be used for the production of probiotics and bacteriocins but need further investigation to optimize the production of bacteriocins


Cintas, L.M., Casaus, M.P., Herranz, C., Nes, I.F. y Hernández P.E. (2001). Review: Bacteriocins of Lactic Acid Bacteria. Food Science and Technology International 7, 281-305.

Dubois, M., Gilles, K., Hamilton, J., Rebers, P. y Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350-356.

García, P., Rodríguez, L., Rodríguez, A. y Martínez, B. (2010). Food biopreservation: promising strategies using bacteriocins, bacteriophages and endolysins. Trends in Food Science & Technology 21, 373-382.

Gonzalez-Olivares, L. G., Jiménez-Guzmán, J., Cruz-Guerrero, A., Rodríguez-Serrano, G., Gómez-Ruíz, L. y García-Garibay, M. (2011). Bioactive peptides released by lactic acid bacteria in commercial fermented milks. Revista Mexicana de Ingeniería Química 10, 179-188.

Gutierrez-Uribe, J.A., Rojas-García, C., García-Lara, S. y Serna-Saldivar, S.O. (2010). Phytochemical analysis of wastewater (nejayote) obtained after lime-cooking of different types of maize kernels processed into masa for tortillas. Journal of Cereal Science 52, 410-416.

Lowry, O.H., Rosebrough, N.J., Farr, A.L., y Randall, J.R. (1951). The protein measurement with the Folin Phenol reagent. Journal of Biological Chemistry 193, 265-275.

Martínez-Bustos, F., Martínez-Flores, H.E., San martín-Martínez, E., Sanchez-Sinencio, F., Chang, Y.K., Barrera-Arellano, D. y Rios, E. (2001). Effect of the components of maize on the quality of masa and tortillas during the traditional nixtamalization process. Journal of the Science of Food and Agriculture 81, 1455- 1462.

Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31, 426-428.

Niño-Medina, G., Carvajal-Millán, E., Lizardi, J., Rascon-Chu, A., Marquez-Escalante, J.A., Gardea, A., Martínez-Lopez, A.L., y Guerrero, V. (2009). Maize processing waste water arabinoxylans: Gelling capability and crosslinking content. Food Chemistry 115, 1286- 1290.

Ochoa, A. y Viniegra-Gónzalez, G. (2009). Temas Selectos de la Cadena Maíz-Tortilla. Mexico, D.F. Impresiones y Diseños de la Universidad Autónoma Metropolitana. 114.

Papagianni, M., Avramidis, N., Filioussis, Dasiou, D. y Ambrosiadis I. (2006). Determination of bacteriocin activity with bioassays carried out on solid and liquid substrates: assessing the factor “indicator microorganism”. Microbial Cell Factories 5, 1-14.

Rodgers, S. (2008). Novel applications of live bacteria in food services: probiotics and protective cultures. Trends in Food Science & Technology 19, 188-197.

Rosentrater, K.A. (2006). A review of corn masa processing residues: Generation, properties, and potential utilization. Waste Management 26, 284-292.

Ruiz-Gutiérrez, M.G., Quintero-Ramos, A., Melendez-Pizarro, C.O., Lardizábal-Gutiérrez, D., Barnard, J., Marquez-Melendez, R. y Talamas-Abbud, R. (2010). Changes in mass transfer, thermal and physicochemical properties during nixtamalization of corn with and without agitation at different temperatures. Journal of Food Engineering 98, 76-83

Todorov, S. D., Vaz-Velho, M., Melo-Franco, B. D. G. y Holzapfel, W. H. (2013). Partial characterization of bacteriocins produced by three strains of Lactobacillus sakei, isolated from salpicao, a fermented meat product from North-West of Portugal. Food Control 30, 111- 121.

Velasco-Martínez, M., Angulo, O., Vazquez- Couturier, D. L., Arroyo-Lara, A., y MonroyRivera, J. A. (1997). Effect of dried solids of nejayote on broiler growth. Poultry Science 76, 1531-153
How to Cite
Ramírez-Romero, G., Reyes-Velazquez, M., & Cruz-Guerrero, A. (2020). STUDY OF NEJAYOTE AS CULTURE MEDIUM FOR PROBIOTICS AND PRODUCTION OF BACTERIOCINS. Revista Mexicana De Ingeniería Química, 12(3), 463-471. Retrieved from

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