LACTOSE-INDUCED EXPRESSION OF RECOMBINANT TURNIP PEROXIDASE IN Escherichia coli

  • R. Pliego-Arreaga
  • C. Regalado
  • A. Amaro-Reyes
  • B.E. García-Almendárez
Keywords: Brassica napus, peroxidase, expression, lactose, fermenter

Abstract

Turnip (Brassica napus L. var. purple top white globe) peroxidases (E.C. 1.11.1.7) may be applied free or immobilized on phenolic compounds degradation in waste water, in addition to its use in diagnostic test kits. The aim of this work was to achieve heterologous expression by lactose-induction of one peroxidase isoform (PodC) using Escherichia coli Rosetta 2 to produce recombinant turnip peroxidase in a fermenter. Recombinant PodC contained in inclusion bodies was refolded under oxidative conditions and purified by IMAC with a yield of 36 mg L-1 of culture. The enzyme showed a specific activity of 1004 ABTS units mg-1 under optimum conditions (pH 6, 45 ◦C). The value of KM for ABTS was 0.38 mM and the catalytic efficiency (kcat=KM) was 5.4×106 s-1 M-1. The use of lactose as inducer and scale up of the fermentation process may effectively decrease the production cost of recombinant PodC, which in turn can be applied for biotechnological applications

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Published
2020-04-02
How to Cite
Pliego-Arreaga, R., Regalado, C., Amaro-Reyes, A., & García-Almendárez, B. (2020). LACTOSE-INDUCED EXPRESSION OF RECOMBINANT TURNIP PEROXIDASE IN Escherichia coli. Revista Mexicana De Ingeniería Química, 12(3), 505-511. Retrieved from http://www.rmiq.org/ojs311/index.php/rmiq/article/view/1524
Section
Biotechnology

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