• J. Marcial Depto. de Biotecnología, Univ. Autónoma Metropolitana-Iztapalapa
  • A.I. Pérez de los Santos Depto. de Biotecnología, Univ. Autónoma Metropolitana-Iztapalapa
  • F.J. Fernández Univ. Autónoma Metropolitana-Iztapalapa
  • G. Díaz-Godínez Universidad Autónoma de Tlaxcala. Centro de Investigación en Ciencias Biológicas.
  • A.M. Montiel-González Universidad Autónoma de Tlaxcala. Centro de Investigación en Ciencias Biológicas.
  • A. Tomasini Depto. de Biotecnología, Univ. Autónoma Metropolitana-Iztapalapa
Keywords: Amylomyces rouxii, aspartic protease, inhibitor protease


The zigomycete Amylomyces rouxii was isolated, in our laboratory, from effluents of paper industries, it was showed that this fungus produces extracellular tyrosinase. In this work, we show that this fungus produces also an extracellular protease. The extracellular protease was partially purified using two-step purification, by (NH4)2SO4 fractionation and DEAE-sepharose anion exchange chromatography with 4.25-fold factor purification. The optimal pH for protease activity, using hemoglobin as substrate, was 3.5. Results from protein purification revealed that this protease is an aspartic protease. Protease activity was inhibited 73% by pepstatin A, a specific inhibitor of acid proteases. Results allow conclude that A. rouxii produce an extracellular aspartic protease that has similar characteristics to aspartic proteases produced by fungi as Mucor and Rhizopus.


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How to Cite
Marcial, J., Pérez de los Santos, A., Fernández, F., Díaz-Godínez, G., Montiel-González, A., & Tomasini, A. (2020). CHARACTERIZATION OF AN ASPARTIC PROTEASE PRODUCED BY Amylomyces rouxii. Revista Mexicana De Ingeniería Química, 10(1), 9-16. Retrieved from