THE EFFECT OF THE RATIO BETWEEN SUBSTRATE CONCENTRATION AND SPECIFIC AREA OF THE SUPPORT ON THE BIOMASS YIELD OF FUNGAL SURFACE CULTURES
Abstract
A parameter,σ0 = S 0α-1 (gcm-2), is defined. Where, S 0, (gcm-3) is the initial substrate concentration and α is the specific area of the solid support. This parameter helps comparing the biomass yields, YX/S , of fungical superficial cultures either with a fixed S 0 value and variable α or with fixed α and variable S 0. A. niger cultures followed the logistic equation with maximal surface density ρAM (gcm-2). Final average thickness, h, was measured by image analysis. The values of h, followed a saturation function of σ0 (R2 = 0.965) with extrapolated value hMAX ~0:4 cm. But the volumetric density ρV =ρAMh-1 was nearly constant ρV ~ 0.046 ±0.005 (gcm-3). The regression Y-1x/S = Y-1 0 +σ0ε-1 (R2 = 0.95) in the range, 5.9 mgcm-2 <σ0 < 62:8 mgcm-2 indicated, Y0 = 0.5, as the maximal biomass yield, and ε=ρv hMAX ~ 0.02 gcm-2, as a measurement of the biosynthetic efficiency. This approach could be useful for the normalization and comparison of screening tests of fungal surface cultures with a variety of solid supports and culture conditions. For example, cultures with different S 0 values but, similar yield coeffient Yx/s.
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