• A. Quintero-Lira
  • R. Hernández-Díaz
  • D. J. Pimentel-González
  • M. E. Rodríguez-Huezo
  • R. G. Campos-Montiel
Keywords: prebiotics, cellulolytic anaerobic bacteria, Aspergillus niger


It has been demonstrated that crude extracts of Aspergillus niger (An) used as additives for animal nutrition have enhanced the digestibility of forages on in vitro assays. In this work the prebiotic component of An that stimulates the growth of a cellulolitic bacterial consortium from ruminal origin (CBCRO) was characterized. Ultrafiltrated fractions of less than 30 kDa, heat treated at 60, 90 and 120 ºC were used. The fractions were obtained by ionexchange chromatography and their average molecular weight was determined by molecular exclusion chromatography. Protein and carbohydrate contents and the infrared spectra were determined on the active fractions. Results showed that all the heat treated ultrafiltrated fractions significantly increased (P<0.05) carboxymethylcellulases’ activities, and protein and acetate productions of the CBCRO. Molecular exclusion chromatography established that the active fraction had a molecular mass of 6.1 kDa, whereas only one active fraction was detected through ion exchange chromatography. Biochemical analysis and infrared spectra data suggest that the prebiotic component of An has a peptidic nature 


Beauchemin, K.A., Colombatto, D., Morgavi, D.P. y Yang, W.Z. (2002). Use of exogenous fibrolytic enzymes to improve feed utilization by ruminants. Journal of Animal Science 81 (Suppl. 2), E37-E47.

Callaway, E.S. y Martin, S.A. (1997). Effects of a of Saccharomyces cerevisiae culture on ruminal bacteria that utilize lactate and digest cellulose. Journal of Dairy Science 80, 2035- 2044.

Campos-Montiel, R. G., Pimentel-Gonzalez, D. J.,Hernández-Fuentes, A. D., Alfaro Rodríguez, R.H. y Viniegra González, G. (2008).Influence of fungal cultures on in vitro ruminal assays with different substrates. Revista Mexicana de Ingeniería Química 7,215-221.

Di Francia, A., Masucci, F., De Rosa, G., Varricchio, M.L. y Proto, V. (2008). Effects of Aspergillus oryzae extract and a Saccharomyces cerevisiae fermentation product on intake, body weight gain and digestibility in buffalo calves. Animal Feed Science and Technology 140 (1-2), 67-77.

Enjalbert, F., Garrett, J.E., Moncoulon, R.,Bayourthe, C. y Chicoteau, P. (1999). Effects of yeast culture (Saccharomyces cerevisiae) on ruminal digestion in non-lactating dairy cows. Animal Feed Science and Technology 76, 195-206.

Fu, C.J., Feldon, E.E.D., Lehmkuhler, J.W. y Kerley, M.S. (2001). Ruminal peptide concentration required to optimize microbial growth and efficiency. Journal of Animal Science 79, 1305-1312.

Harper, E.G., Welch, R.P., Contreras-Lara, D., Chang, J.S. y Calza, R.E. (1996). The effect of Aspergillus oryzae fermentation extract on the anaerobic fungi Neocallimastix frontalis EB 188, Piromyces communis DC 193 and Orpinomycs ssp RW206: generalized effects and component analysis. Applied Microbiology and Biotechnology 45, 817-821.

Hernández-Díaz, R., Pimentel-González, D.J., Figueira, A.C., Viniegra-González, G. y Campos-Montiel, R.G. (2008). Influence of an aerobic fungus grown on solid culture on ruminal degradability and on a mixture culture of anaerobic cellulolytic bacteria. Journal of Animal Physiology and Animal Nutrition DOI: 10.1111/j.1439-0396.2008.00912.x

Lee, S.S., Ha, J.K. y Cheng, K.J. (2000). Influence of an anaerobic fungal culture administration on in vivo ruminal fermentation and nutrient digestion. Animal Feed Science and Technology 88, 201-217.

Lee, S.S., Choi, C.K., Ahn, B.H., Moon, Y.H., Kim, C.H. y Ha, J.K. (2004). In vitro stimulation of rumen microbial fermentation by a rumen anaerobic fungal culture. Animal Feed Science and. Technology 115, 215–226.

Malley, D.F. (1998). Near-infrared spectroscopy as a potencial method for routine sediment analysis in improve rapidity and efficiency. Water Science and Technology 37, 181-188.

Miller, G.L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31, 426-428.

Oeztuerk, H., Schroeder, B., Beyerbach, M. y Breves, G. (2005). Influence of living and autoclaved yeast Saccharomyces boulardii on in vitro ruminal microbial metabolism. Journal of Dairy Science 88, 2594-2600.

Reeves, J.B. (2001). Mid-infrared diffuse reflectance spectroscopy for the analysis of poultry manures. Journal of Agricultural and Food Chemistry 49, 2193-2197.

Ruíz-Leza, H.A., Rodríguez-Jasso, R.M., Rodríguez- Herrera, R., Contreras-Esquivel, J.C. y Aguilar, C.N. (2007). Diseño de biorreactores para fermentación en medio sólido. Revista Mexicana de Ingeniería Química 6 (1), 33-40.

SAS. (1990). SAS/STAT User´s Guide (Version 6), 4Th ed. Statistical Analysis Systems Institute, Cary, NC, USA.

Schmidt, J.A., Albright, S., Calza, G.M. y Calza, R.E. (2004). Characterization of Aspergillus oryzae fermentation extract effects on the rumen fungus Neocallimastix frontalis EB 188. Part 2. Carbon source utilization and effects on zoospore production. Applied Microbiology and Biotechnology 63, 431-437.

Tapingkae, W., Yachai, M., Visessanguan, W., Pongtanya, P. y Pongpiachan, P. (2008). Influence of crude xylanase from Aspergillus niger FAS128 on the in vitro digestibility and production performance of piglets. Animal Feed Science and Technology 140, 125–138.

Tricarico, J.M., Abney, M.D., Galyean, M.L., Rivera, J.D., Hanson, K.C., McLeod, K.R. y Harmon, D.L. (2007). Effects of a dietary Aspergillus oryzae extract containing α-amylase activity on performance and carcass characteristics of finishing beef cattle. Journal of Animal Science 85, 802-811.

Welch, R.D., Tsai, K.P., Harper, E.G., Chang, J.S. y Calza, R.E. (1996). The effect of Aspergillus oryzae fermentation extract on the anaerobic fungus Neocallimastix frontalis EB 188 effects on physiology. Applied Microbiology and Biotechnology 45, 811-816.
How to Cite
Quintero-Lira, A., Hernández-Díaz, R., Pimentel-González, D. J., Rodríguez-Huezo, M. E., & Campos-Montiel, R. G. (2020). CHARACTERIZATION OF THE PREBIOTIC COMPONENT OF Aspergillus niger THAT STIMULATES THE CELULLOLYTIC BACTERIA OF RUMINAL ORIGIN. Revista Mexicana De Ingeniería Química, 8(1), 51-55. Retrieved from

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