GENERIC APPLICATION OF POLYETHYLENE GLYCOL – SALT AQUEOUS TWOPHASE SYSTEMS FOR THE DEVELOPMENT OF PROCESSES TO BIOLOGICAL PRODUCTS PRIMARY RECOVERY

  • J. Benavides
  • M. Rito-Palomares
Keywords: aqueous two-phase systems, primary recovery, biological products

Abstract

The generic application of Aqueous Two-Phase Systems (ATPS) polyethylene glycol (PEG) – salt was evaluated as a practical strategy for the development of bioprocesses for the primary recovery of biological products. Four experimental models were used: B-phycoerythrin (BFE) from Porphyridium cruentum, C-phycocyanin (CFC) from Spirulina maxima, double layered rotavirus-like particles (dlRLP) produced in High FiveTM insect cells, and lutein from Chlorella protothecoides. Influence of the system parameters (polymer molecular weight, MW PEG; tie line length, TLL; volume ratio, VR; system pH) upon partition behavior of the selected experimental models. Process conditions for the primary recovery of the products of interest using ATPS were established. Such conditions result in processes with reduced number of stages, which favored high yields. The influence of system parameters upon partition behavior of the four experimental models was correlated with their physicochemical properties, which allowed establishing general rules that facilitate the predictive development of processes for the primary recovery of biological products using SDFA PEG – salt.

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Published
2020-06-12
How to Cite
Benavides, J., & Rito-Palomares, M. (2020). GENERIC APPLICATION OF POLYETHYLENE GLYCOL – SALT AQUEOUS TWOPHASE SYSTEMS FOR THE DEVELOPMENT OF PROCESSES TO BIOLOGICAL PRODUCTS PRIMARY RECOVERY. Revista Mexicana De Ingeniería Química, 7(2), 99-111. Retrieved from http://www.rmiq.org/ojs311/index.php/rmiq/article/view/1812
Section
Biotechnology

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