THE ABTS●+ AN OXIDANT AGENT OF DIFFERENT CHEMICAL COMPOUNDS AND ITS RECYCLING PROCESS BETWEEN LACCASE AND SUBSTRATE

  • M. Solís-Oba Centro de Investigación en Biotecnología Aplicada del Instituto Politécnico Nacional
  • E. Bárzana Facultad de Química, Universidad Nacional Autónoma de México
  • M. García-Garibay Departamento de Biotecnología, Universidad Autónoma Metropolitana Unidad Iztapalapa
  • G. Viniegra-González Departamento de Biotecnología, Universidad Autónoma Metropolitana Unidad Iztapalapa
Keywords: ABTS, recycling, oxidation, dyes, lacction

Abstract

The commercial enzyme laccase cloned from Myceliophtera termophila and expressed in Aspergillus oryzae was used to produce an active, stable and oxidized form of the chemical mediator ABTS (2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)) in order to test the feasibility of using the monocation ABTS + as a recyclable intermediate for the oxidation of a variety of aromatic chemicals in the absence of the enzyme. It was shown that ABTS +, by itself, oxidized, indigo blue, tannic and galic acids, brilliant blue G, Coomassie blue,
orange 7 and p-cresol. Oxidation was assessed by important changes in their visible absorption spectra. It was also shown that oxidation of these compounds by a mixture of ABTS and laccase can be done in a cyclic manner, such a way the oxidation rate was 94 times higher for indigo, 17 times for brilliant blue G, 34 times for orange 7 and 5 times higher for p-cresol compared with using the mediator or the laccase alone. Those results show the feasibility of a recycling scheme for the final oxidation of a wide variety of aromatic compounds in which the enzyme is not directly in contact with the compound but through the intermediate ABTS +.

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Published
2020-08-17
How to Cite
Solís-Oba, M., Bárzana, E., García-Garibay, M., & Viniegra-González, G. (2020). THE ABTS●+ AN OXIDANT AGENT OF DIFFERENT CHEMICAL COMPOUNDS AND ITS RECYCLING PROCESS BETWEEN LACCASE AND SUBSTRATE. Revista Mexicana De Ingeniería Química, 6(3), 275-281. Retrieved from http://www.rmiq.org/ojs311/index.php/rmiq/article/view/1957
Section
Biotechnology