Agave salmiana syrup improves the production of recombinant human interleukin-2 in Escherichia coli

Keywords: codon optimization, fructose, NaCl inducer, recombinant protein, synthetic gene.


The expression of heterologous proteins in Escherichia coli is strongly affected by the type of carbon source used. In this work, the expression of a synthetic codon-optimized gene of human interleukin-2 in E. coli BL21-SI, carrying plasmid pET12a-hIL2 is presented. Glucose, fructose or Agave syrup from Agave salmiana, were used as carbon sources for production of recombinant human IL-2 (rhIL-2) in 1.5-L bioreactor aerobic cultures using mineral medium. Codon optimization of the native hIL-2 gene eliminated the presence of 35 rare codons for E. coli, and improved the codon usage up to 76% compared with the native gene sequence. Cultures using 10 g/L glucose showed the lowest production of rhIL-2, and in contrast, cultures using fructose improved the production of rhIL-2 1.9-times. The utilization of fructose from Agave syrup enhanced the rhIL-2 production 3.9-times, reaching 103.42±6.61 mgIL-2/L. The specific rhIL-2 production rate (5.52±0.33 mgIL-2/gDCW·h) using Agave syrup was also the highest. These results indicate that Agave syrup stimulates the production of rhIL-2 and it is an inexpensive alternative carbon source. This research abilities the potential use the Agaves to produce alternative and valuable biotechnological products instead the alcoholic beverages.


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How to Cite
Balderas-Hernández, V., Medina-Rivero, E., Barba-De la Rosa, A., & De León-Rodríguez, A. (2020). Agave salmiana syrup improves the production of recombinant human interleukin-2 in Escherichia coli. Revista Mexicana De Ingeniería Química, 20(1), 399-412.

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