FRONTAL CHROMATOGRAPHY OF PLASMIDS: PARAMETERS ESTIMATION AND SIMULATION
A transport model that considers external film resistance, finite kinetic rate and column dispersed flow was used to describe the performance of a Q Sepharose ion-exchange column in plasmid pMa5 purification. The system kinetic and thermodynamic constants were obtained from a experimental system from the literature. The transport parameters were estimated using mathematical correlations for packed-bed columns and plasmids. The simulation studies were conducted using the numerical method of lines. This approach allowed to examine different strategies of design of the separation system, by means of parametric analysis and by system scale up.
Danckwerts, P.V. (1986). Continuous flow systems. Distribution of residence times. Chemical Engineering Science 2, 219-230.
Ferreira, G.N.M., Cabral, J.M.S. y Prazeres, D.M.F. (2000). Studies on the batch adsorption of plasmid DNA onto anion exchange chromatographic supports. Biotechnology Progress 16, 416-424.
Foo, S.C. y Rice, R.G. (1975). On the prediction of ultimate separation in parametric pumps. American Institute of Chemical Engineers Journal 21, 1149-1158.
He, L. y Niemeyer, B. (2003). A novel correlation for protein diffusion coefficients based on molecular weight and radius of gyration. Biotechnolology Progress 19, 544-548.
Kempe, H., Axelsson, A., Nilsson, B. y Zacchi, G. (1999). Simulation of chromatographic processes applied to separation of proteins. Journal of Chromatography A 846, 1-12.
Prazeres, D.M.F., Ferreira, G.N.M., Monteiro, G.A., Cooney, C. y Cabral, J.M.S. (1999). Large-scale production of pharmaceuticalgrade plasmid DNA for gene therapy: problems and bottlenecks. Trends in Biotechnology 17, 169-174.
Schalk, I.; Hegger, P.M.; Jongen, J.M. (2001). Gene therapeutics and DNA vaccines, quality and regulatory aspects. National Institute of Public Health and Environment. RIVM report 605200 001.
Varley, D. L., Hitchcock, A. G., Weiss, A.M.E., Horler, W.A., Cowell, R., Peddie, L., Sharpe, G.S., Thatcher, D.R. y Hanak, J.A.J. (1999). Production of plasmid DNA for human gene therapy using modified alkaline cell lysis and expanded bed anion exchange chromatography. Bioseparations 8, 209-217
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