SYNTHESIS OF CLA-ENRICHED TRIACYLGLYCEROLS BY ENZYMATIC POLYESTERIFICATION IN A SOLVENT-FREE MEDIUM
Conjugated linoleic acid (CLA) is a term used to designate a mixture of positional and geometrical isomers of linoleic acid. Several of these isomers have been associated with important biological roles, including anti carcinogenic activity. Basically, the natural sources of CLA are dairy and meat products. However, its presence in such foods is in very small quantities. The present work was undertaken to evaluate the effect of temperature (40, 50, 60 and 70°C), molar ratio of substrates (3:1 and 4:1, free fatty acid: glycerol), and two commercially available biocatalysts [Candida antarctica fraction B (Chyrazyme L-2), and Rhizomucor miehei (IM-60)], on the rate of production of triacylglycerols (TAG) from glycerol and CLA via enzyme-mediated polyesterification reactions. The formation of acylglycerols and the consumption of the free fatty acids were monitored by periodic withdrawal of samples for analysis by HPLC. Best treatments were selected by the Tukey’s multiple comparison test (p<0.05). The conditions which produced maximum synthesis of TAG (76% ±2.7 moles of the original fatty acid were esterified to form TAG), were those in which free fatty acid was used in molar excess (4:1 mole ratio of CLA to glycerol) and high temperatures (60 and 70°C) were employed. For these conditions, there were no significant differences between the results obtained with either commercial enzyme. For both enzymes, a linear relationship was observed between the production of the TAG and the reaction temperature
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