BIOENGINEERING STRATEGIES FOR THE PRIMARY RECOVERY OF BIOLOGICAL PRODUCTS

  • M. Cisneros-Ruiz
  • M. Rito-Palomares
Keywords: bioengineering strategies, recovery, process integration, process intensification

Abstract

The increasing interest of the pharmaceutical companies to develop efficient and scale-up processes, that allow them to rapidly bring new products to the market, has forced them to develop new bioengineering strategies. One of the current trend is to exploit bioprocess integration and intensification approaches for the development of recovery and purification processes for biological products, particularly proteins. This article presents some cases of the results of the practical application of these approaches, using the techniques of aqueous two phase system and expanded bed adsorption. The experimental cases involve recovery of intracellular protein from baker´s yeast, in situ recovery of 6- pentyl-α-pyrone (coconut-like aroma compound) produced by Trichoderma harzianum, the development of a prototype process for the recovery of c-phycocyanin from Spirulina maxima and a new approach for the processing and recovery of inclusion bodies. It is expected that in the future the application of these strategies will facilitate the development of recovery systems for products of high commercial value and the optimization of already existing processes, attracting the attention of the industry for commercial applications.

References

Bailey S. M., Blum P. H. y Meagher M. (1995). Improved homogenization of recombinant Escherichia coli following pretreatment with guanidine hydrochloride. Biotechnology Progress 11, 533-539.

Bierau H., Zhang Z. y Lyddiatt A. (1999). Direct process integration of cell disruption and fluidised bed adsorption for the recovery of intracellular proteins. Journal of Chemical Technology and Biotechnology 74, 208-212.

Choe W., Clemmitt R., Rito-Palomares M., Chase H. y Middelberg A. P. J. (2002). Bioprocess intensification: a radical new process for recovering inclusion body protein. Transactions of Institution of Chemical Engineers 80, 45-50.

Falconer R. J., O’Neill B. K. y Middelberg A. P. J. (1997). Chemical treatment of Escherichia coli: I. Extraction of intracellular protein from uninduced cells. Biotechnology and Bioengineering 53, 458-463.

Falconer R. J., O’Neill B. K. y Middelberg A. P. J. (1998). Chemical treatment of Escherichia coli. II. Direct extraction of recombinant protein from cytoplasmic inclusion bodies in intact cells. Biotechnology and Bioengineering 57, 381-386.

Falconer R. J., O’Neill B. K. y Middelberg A. P. J. (1999). Chemical treatment of Escherichi coli. III. Selective extraction of a recombinant protein from cytoplasmic inclusion bodies in intact cells. Biotechnology and Bioengineering 62, 455-460.

Forman S. M., deBernardez E. R., Feldberg R. S. y Swartz R. W. (1990). Crossflow filtration for the separation of inclusion bodies from soluble proteins in recombinant Escherichia coli cell lysate. Journal of Membrane Science 48, 263-279.

Hart R. A., Lester P. M., Reifsnyder D. H., Ogez J. R. y Builder S. E. (1994). Largescale in situ isolation of periplasmic IGFI from Escherichia coli. Biotechnology 12, 1113-1117.

Hart R. A., Ogez J. R. y Builder S. E. (1995). Use of multifactorial analysis to develop aqueous two-phase systems for isolation of non-native IGF-I. Bioseparation 5, 113-121

Herrera A., Boussiva S., Napoleone V. y Holberg A. (1989). Recovery of cphycocyanin from the cyanobacterium Spirulina maxima. Journal of Applied Phycology 1, 325-331

Ling Y., Wong H. H., Thomas C. J., Williams D. R. y Middelberg A. P. J. (1997). Pilot scale extraction of PHB from recombinant E. coli by homogenization and centrifugation. Bioseparation 7, 9-15.

Lyddiatt, A. (2002). Process chromatography: current constraints and future options for the adsorptive recovery of bioproducts. Current Opnion in Biotechnology 13, 95-103.

Meagher M. M., Barlett R. T., Rai V. R. y Khan F. R. (1994). Extraction of rIL-2 inclusion bodies from Escherichia coli using cross-flow filtration. Biotechnology and Bioengineering 43, 969-977.

Rito-Palomares M. (2002). The practical application of aqueous two-phase processes for the recovery of biological products. Journal of Microbiology and Biotechnology 12, 535-543.

Rito-Palomares M. y Lyddiatt A. (2002). Process integration using aqueous twophase partition for the recovery of intrecellular proteins. Chemical Engineering Journal 87, 313-319.

Rito-Palomares M. y Middelberg A. P. J. (2002). Aqueous two-phase systems for the recovery of a recombinant viral coat protein from Escherichi coli. Journal of Chemical Technology and Biotechnology 77, 1025-1029.

Rito-Palomares M., Nuñez L. y Amador D. (2001). Practical application of aqueous two-phase systems for the development of a prototype process for c-phycocyanin recovery from Spirulina maxima. Journal of Chemical Technology and Biotechnology 76, 1273-1280.

Rito-Palomares, M. (2004). Practical application of aqueous two-phase partition to process development for the recovery of biological products. Journal of Chromatography B. 807, 3-11.

Rito-Palomares, M., Negrete, A., Miranda, L., Flores, C., Galindo, E., y Serrano- Carreón, L. (2001). The potential application of aqueous two-phase systems for in situ recovery of 6-pentyl- α-pyrone produced by Trichoderma harzianum. Enzyme and Microbiology Technology 28, 625-631.

Thömmes J., Halfar M., Gieren H., Curvers S., Takors R., Brunschier R. y Kula M.- R. (2001). Human chymotrypsinogen B production from Pichia pastoris by integrated development of fermentation and downstream processing Part 2. Protein recovery. Biotechnology Progress 17, 503-512.

Wong H. H., O´Neill B. K. y Middelberg A. P. J. (1997). A mathematical model for Escherichia coli debris size reduction during high pressure homogenization based on grinding theory. Chemical Engineering Science 52, 2883-2890.

Zijlstra G. M., de Gooijer C. D. y Tramper J. (1998). Extractive bioconversions in aqueous two phase systems. Current Opinion in Biotechnology 9, 171-176
Published
2020-10-10
How to Cite
Cisneros-Ruiz, M., & Rito-Palomares, M. (2020). BIOENGINEERING STRATEGIES FOR THE PRIMARY RECOVERY OF BIOLOGICAL PRODUCTS. Revista Mexicana De Ingeniería Química, 4(1), 131-139. Retrieved from http://www.rmiq.org/ojs311/index.php/rmiq/article/view/2094

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