Rice husk (Oryza sativa) as support in the immobilization of yeast cells

  • D. Trujillo-Ramírez
  • M.G. Bustos-Vázquez Doctora
  • L.V. Rodríguez-Durán
  • R. Torres-de los Santos
Keywords: rice husk, cell immobilization, yeast cells, acid and alkaline pretreatment.

Abstract

The present study aims to evaluate the rice husk (Oriza sativa; RH), using H2SO4 and NaOH (concentration of 2% v/v, solid to liquid ratio 1:6 and temperature and reaction time of 121 °C and 40 min, respectively, for both treatments) as pretreatment to determine if this material can be used as support in the immobilization of Saccharomyces cerevisiae cells. Fermentation kinetics were evaluated in cell buffer solution solid to liquid ratio 1:20 and sampling was performed every 8 h for 32 h. The highest cellular retention of RH pretreated with H2SO4 (PretAcid) was at 24 h (98.35 mg g–1), while RH pretreated with NaOH (PretAlka), was at 16 h (63.21 mg g–1), meanwhile, the treatment that was not subjected to chemical treatment (Control), was at 24 h (8.56 mg g–1). On the other hand, the maximum support efficiency was reached at 36 h in the order of 44.99, 23.25 and 23.43 % for PretAcid, PretAlka and Control, respectively. These results indicate that due to the structural modification that RH underwent during chemical pretreatments, may be an ideal substrate for the adsorption of yeast cells.

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Published
2021-11-22
How to Cite
Trujillo-Ramírez, D., Bustos-Vázquez, M., Rodríguez-Durán, L., & Torres-de los Santos, R. (2021). Rice husk (Oryza sativa) as support in the immobilization of yeast cells. Revista Mexicana De Ingeniería Química, 21(1), Bio2558. https://doi.org/10.24275/rmiq/Bio2558
Section
Biotechnology