MORPHOGENESIS AND SECONDARY METABOLITES PRODUCTION IN THE MEDICINAL PLANT Castilleja tenuiflora Benth. UNDER NITROGEN DEFICIENCY AND STARVATION STRESS IN A TEMPORARY IMMERSION SYSTEM

  • J.A. Cortes-Morales
  • A.R. López-Laredo
  • A. Zamilpa
  • K. Bermúdez-Torres
  • J.L. Trejo-Espino
  • G. Trejo-Tapia
Keywords: abiotic stress, Aucubin, nitrogen starvation, phenylethanoid glycosides, RITA® bioreactor

Abstract

Castilleja tenuiflora Benth. is a medicinal plant that synthesizes polyphenolic compounds such as phenylethanoid glycosides (PhGs), flavonoids, lignans, and iridoid glycosides with pharmacological activities. In vitro culturing of plants combined with a stress as nutrient deficiency has been shown to be a good biotechnological strategy to stimulate secondary metabolism. Our aim was to evaluate the responses of C. tenuiflora plants to stress by nitrogen (N) deficiency and starvation in a temporary immersion system. The treatments were plants grown in B5 liquid medium with 25.74 mM (control), 1.32, 0.66 (deficiency) and 0 mM (starvation) N concentration. Matter and shoot multiplication rate decreased over 40%, chlorophylls content decreased, roots length increased, and leaves showed chlorosis. In deficiency conditions (0.66 mM N), PAL enzyme was more active and total phenolic compounds were enhanced. At day 21: maximum concentration of verbascoside, isoverbascoside and aucubin was observed in 0.66, 1.32 and 0 mM N treatments (283 ± 0.5, 90 ± 0.3 and 12.5 ± 0.1 mg g-1 DM, respectively) and presence of anthocyanins was evident in stems. Under N deficiency, the first defense response is an oxidative burst, PhGs and iridoids are powerful antioxidants; thereby, the increase of these compounds and de novo synthesis of anthocyanins relief oxidative damage. Our results show that in vitro culture in a temporary immersion system combined with N deficiency is an excellent method to increase secondary metabolites in C. tenuiflora plants.

Published
2018-04-15
How to Cite
Cortes-Morales, J., López-Laredo, A., Zamilpa, A., Bermúdez-Torres, K., Trejo-Espino, J., & Trejo-Tapia, G. (2018). MORPHOGENESIS AND SECONDARY METABOLITES PRODUCTION IN THE MEDICINAL PLANT Castilleja tenuiflora Benth. UNDER NITROGEN DEFICIENCY AND STARVATION STRESS IN A TEMPORARY IMMERSION SYSTEM. Revista Mexicana De Ingeniería Química, 17(1), 229-242. https://doi.org/10.24275/uam/izt/dcbi/revmexingquim/2018v17n1/Cortes
Section
Biotechnology

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